File S1 - Correlations between Transmembrane 4 L6 Family Member 5 (TM4SF5), CD151, and CD63 in Liver Fibrotic Phenotypes and Hepatic Migration and Invasive Capacities

Contains the files: Figure S1. Transwell migration analysis was performed for Chang-TGFβ1 cells transfected with the indicated shRNAs or plasmids. The bottom chamber was filled with 10% FBS/DMEM-H and cells were loaded to upper chamber. After 18 h, cells migrated to the bottom surface of the filter was stained and imaged. Representative images more than at least 5 images were considered for counting of migrated cells in each experimental condition. Images of a representative experimental set were shown for each experimental condition. Data represent three independent experiments. Figure S2. Transwell migration analysis was performed for Chang-TGFβ1 cells transfected with the indicated shRNAs or plasmids. The bottom chamber was filled with 10% FBS/DMEM-H and cells were loaded to upper chamber. After 18 h, cells migrated to the bottom surface of the filter was stained and imaged. Representative images more than at least 5 images were considered for counting of migrated cells in each experimental condition. Images of a representative experimental set were shown for each experimental condition. Data represent three independent experiments. Figure S3. Chang-TGFβ1 cells transfected with shRNA against TM4SF5 (shTM4SF5) or CD151 (shCD151) were reseeded on coverglasses precoated with Oregon Green® 488-conjugated gelatin and incubated for 18 h in a CO2 incubator, before staining actin and then visualizing fluorescent-gelatin degradation (dark). The dark-spotted ECM-degraded area from images more than 5 random areas were saved and a representative set was shown. Data represent three independent experiments. Figure S4. Chang-TGFβ1 cells transfected with shRNA against TM4SF5 (shTM4SF5) or CD151 (shCD151) without or with TM4SF5 or CD151 cDNA plasmids were reseeded on coverglasses precoated with Oregon Green 488-conjugated gelatin and incubated for 18 h in a CO2 incubator, before staining actin and then visualizing fluorescent-gelatin degradation (dark). The dark-spotted ECM-degraded area from images more than 5 random areas were saved and a representative set was shown. Data represent three independent experiments.
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