Airway administration of flagellin regulates the inflammatory response to Pseudomonas aeruginosa

Here, we aim to understand the role of P38 in the airway epithelial cells response to flagellin. We report the gene expression profile of pig airway epithelial cells cultured under air-liquid conditions, pre-incubated or not with the P38 inhibitor SB203580 (20 µM, Tocris Bioscience) for 1 h and then stimulated with 100 ng/ml of a mutated flagellin (FliCΔ174-400) for 2h or 24h. Our data show that flagellin stimulation induce the expression of pro-inflammatory cytokines mainly through the NFkB pathway, with little impact of P38 inhibition in the cells response. RNA-seq analysis of airway epithelial cells pre-incubated (SB) or not (DMSO) with the P38 inhibitor SB203580 and stimulated with 100 ng/ml of flagellin for 2 or 24h.