IGF1R signaling primes epithelial cancer cells to induce epithelial-mesenchymal plasticity by upregulating integrin αV in cutaneous squamous cell carcinoma

Asses the global (phospho) protein profile of cancer cell lysates of three cell biological replicates of each stage of our model of skin SCC progression (full epithelial, plastic EpCAM-positive, plastic EpCAM-negative, and full mesenchymal) isolated from mouse skin SCCs by FACS sorting. The main research question in this experiment using pTyrIP, total protein lysate, and TiOx profiling is to check for differences in full epithelial vs plastic EpCAM(+) cancer cells, and plastic EpCAM(+) vs plastic EpCAM(-) cancer cells. With this information we would like to identify kinase signalling pathways that are induced in each of these stages and that may promote SCC progression, in order to design strategies to block tumor progression.