Taurocholate acid signals a specific gene expression reprogramming affecting amino-acids and nucleotides metabolisms

Enterococcus faecalis is a natural inhabitant of the human gastrointestinal tract. In a healthy physiological state of the gut (eubiosis), E. faecalis is a subdominant species in the intestinal microbiota. When the intestinal homeostasis is disrupted (dysbiosis), it becomes dominant species and can cause infections. The taurocholate bile acid (TCA) becomes prominent during dysbiosis. This experiment aimed to better understand how E. faecalis adapts to TCA. We showed that TCA reprograms genes involved in the pool management of amino acids and nucleotides. Overall design: Impact of TCA on E. faecalis gene expression. Samples were sequenced at different time points after addition of 2 mM TCA (0.1% final).