Detection of Iris yellow spot virus (IYSV) and Tomato spotted wilt virus (TSWV) in Datura stramonium plants.

*D. stramonium plants infected with IYSV or TSWV alone, or co-inoculated were tested for the presence of IYSV non structural protein (NSs) using direct antigen coated-enzyme linked immunosorbent assay, and the IYSV and TSWV nucleocapsid (N) proteins using double antibody sandwich enzyme-linked immunosorbent assay. N/A: not applicable as the antisera are specific to homologous antigens and do not cross react. The A405 values of uninfected (healthy) controls ranged from0.12 for 0.14 for all three antisera.