Archaic humans have contributed to large-scale variation in modern human T cell receptor genes

The dataset contains the repertoire sequencing data from 45 adult volunteers.
For
each case 4 separate TCR repseq libraries were constructed after cDNA
synthesis using a UMI containing primer located in the constant exon of
the TRA, TRD, TRB and TRG genes respectively. In each case a 5’
multiplex set of primers specific for the leader regions of the V genes
of either TRA, TRD, TRB or TRG genes was used along with a universal
reverse primer, introduced during the cDNA synthesis step to produce the
gene specific repseq library.
The
libraries were sequenced using the Illumina 2 x 300 cycle MiSeq system
at the Karlsson Hedestam Laboratory at the Department of Microbiology,
Tumor and Cell Biology at Biomedicum, Karolinska Institutet, Stockholm.

Terms for access
The
datasets are only to be used for research that is seeking to advance
the understanding of the influence of TCR genetic variation on human
biology.