Hippoglossus hippoglossus SNP data for coverage-based sex association

Changes in the genetic mechanisms that control sexual determination have occurred independently across the tree of life, and with exceptional frequency in teleost fishes. To investigate the genomic changes underlying the evolution of sexual determination, we sequenced a chromosome-level genome, multi-tissue transcriptomes, and population genomic data for the Atlantic Halibut (Hippoglossus hippoglossus), which has an XY/XX sex determination mechanism and has recently diverged from the Pacific Halibut (Hippoglossus stenolepis), which has a ZZ/ZW system. We used frequency and coverage-based population genomic approaches to identify a putative sex-determining factor, GSDF. We characterized regions with elevated heterozygosity and linkage disequilibrium indicating suppression of recombination across a recently formed sex chromosome. We detected testis-specific expression of GSDF, the sequence for which was highly conserved across flatfish. Based on evidence from genome-wide association, cove...