ChIP-seq of Bacillus subtilis wild type and RNase J1 null strains

RNase J1 is the first nuclease with 5'-3' exonuclease activity in bacteria and plays an important role in the maturation and degradation of mRNA. RNase J1 could also play a role in transcription termination of aberrant complexes. RNase J1 could bind to nascent RNA in such complexes, degrade the nascent RNA, and upon catching up with RNA polymerase (RNAP) dissociate the complex. Similar model was showed in eukaryotes. We did ChIP-seq to confirm our hypothesis. Complementary RNA-seq data have also been deposited at ArrayExpress under accession number E-MTAB-5660 ( https://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-5660 ).