Diverse 3D chromatin architecture of medium and large plant genomes

The spatial organization of the eukaryote genome plays an important role in the regulation of gene expression. Our understanding of the 3D architecture of the plant nucleus is largely limited to the model plant Arabidopsis, which has a gene dense and repeat poor genome. In this study, we have used chromosome conformation capture to examine the mesophyll cell nuclei of maize, tomato, sorghum, foxtail millet and rice with genome sizes ranging from 0.4 Gb to 2.4 Gb. We also profiled their DNA methylation, histone modifications, accessible chromatin and gene expression.