Real-time quantitative PCR analysis of human corneal cell types

Primary human limbal epithelial cells, corneal fibroblasts and a human neuronal cell line SY-SY5Y were incubated with recombinant human galectin-3 or BSA control and mRNA collected for neurotrophic factor mRNA expression. qPCR gene expression profiling. Cultures from three different donors (primary cells) or cells plated on three separate days (cell line) were used to collect mRNA. Equal amounts of total RNA from each donor were used for PCR arrays.