FTO alleviates psoriasis-like dermatitis through the regulation of FN1

FTO was found to inhibit keratinocyte inflammation and proliferation in both in vitro and in vivo settings, independent of m6A demethylase function. RNA-seq was used to identify fibronectin (FN1,FN) as a potential target for FTO. FTO does not rely on demethylase activity to decrease FN1 expression. The anti-inflammatory and anti-proliferative effects of FTO on psoriasis partly depend on FN1. FTO may regulate the skipping of EDA exon in FN1. Overall design: RNA sequencing was conducted on HaCaT cells that were either overexpressing an empty vector, wild-type FTO, or a mutant type that disrupts the demethylase activity.