IgH targeted reads from CD138+ plasma cells using the Ion S5, MiSeq, and Oxford Nanopore sequencing platforms

Gzipped fastq file of reads from sequencing of CD138+ plasma cells (multiple myeloma) using Ion S5, MiSeq, and Nanopore platforms. The patient sample has been characterized in a previous paper (pt. 1):Cédile O, Hansen MH, Dahlmann SK, Kristensen TK, Abildgaard N, Nyvold CG. Reproducibility of low-level residual myeloma immunoglobulin detection using ultra-deep sequencing. Exp Hematol. 2023 Mar-Apr;119-120:14-20. doi: 10.1016/j.exphem.2023.01.002. Epub 2023 Jan 25. PMID: 36708872.The Oxford Nanopore reads are converted to fastq-like format, where only the sequences have been preserved but is compatible with the swigh-score analysis tools. This tool can be downloaded from http://github.com/marcus-hoy-hansen/swigh-score and has been tested on Ubuntu version 18 to 22.

本数据集为经gzip压缩的FASTQ（fastq）格式文件，包含通过Ion S5、MiSeq及纳米孔（Nanopore）测序平台对CD138阳性浆细胞（CD138+ plasma cells）、多发性骨髓瘤（multiple myeloma）进行测序得到的读段。该患者样本已在既往研究（第一篇）中完成表征：Cédile O、Hansen MH、Dahlmann SK、Kristensen TK、Abildgaard N、Nyvold CG. 基于超深度测序的低水平残留骨髓瘤免疫球蛋白检测的可重复性. 《实验血液学（Experimental Hematology）》, 2023年3-4月;119-120卷:14-20页. DOI: 10.1016/j.exphem.2023.01.002. 2023年1月25日在线发表. PMID: 36708872. 牛津纳米孔（Oxford Nanopore）测序得到的读段已被转换为类FASTQ格式，仅保留序列信息，但可兼容swigh-score分析工具。该工具可从http://github.com/marcus-hoy-hansen/swigh-score下载，且已在Ubuntu 18至22版本中完成测试。