Database of Triatoma vitticeps occurrences and Trypanosoma cruzi infection status in Espírito Santo State, Brazil (2010–2020).

Data were compiled from UFES, Fiocruz (LABTRIP), and Nemes/SESA records. Triatomines were identified following Lent & Wygodzinsky (1979) and examined for T. cruzi-like flagellates. Of 2,527 specimens, 1,530 (60.55%) were infected; 95 DTUs were identified: 36 samples corresponded to DTU TcII, 5 to DTU TcIII, 40 to DTU TcIV, and 14 to Z3. DTUs were identified using multiplex PCR of the mini-exon gene, followed by PCR-RFLP assays targeting 1f8/Alw21I (TcII) and histone 3 (H3)/AluI (TcIII and TcIV). Mixed infections involving TcII and TcIII/TcIV could not be distinguished with this method. Complementary Nemes/SESA data were included to ensure full state-wide coverage. (XLSX)