Metagenomic Analysis of Rhizosphere Microbial Communities Along a Bamboo Expansion Gradient in Subtropical Forests of Jinggangshan, China

This dataset contains rhizosphere metagenomic sequences from a study investigating the effects of Moso bamboo (Phyllostachys edulis) expansion on rhizosphere microbial communities in subtropical forests. Rhizosphere soil samples were collected from 36 plots representing three forest types: pure bamboo forest (PBF), mixed bamboo forest (MBF), and non-expanded forest (NEF) at the Jinggangshan NationSal Nature Reserve, China. For each plot, composite rhizosphere soil samples were collected from the root zone. Total genomic DNA was extracted from rhizosphere samples using the OMEGA Mag-Bind Soil DNA Kit, and shotgun metagenomic libraries were constructed using the Illumina TruSeq Nano WDNA LT Library Preparation Kit. Sequencing was performed on an Illumina NovaSeq platform with PE150 strategy. The dataset provides insights into rhizosphere microbial community composition and functional diversity across different stages of bamboo expansion in subtropical forest ecosystems.