New insights into the molecular actions of Grosheimin, Costunolide, a- and ß- Cyclocostunolide on primary cilia structure and Hedgehog signaling

Human retinal pigment epithelial (RPE) cells were treated with the sesquiterpene lactones a-cyclocostunolide (a-C) or Grosheimin to investigate their effects on primary cilium structure and associated signaling pathways. Sesquiterpene lactones (SLs) are plant-derived metabolites known for diverse pharmacological activities. Previous studies have shown that certain SLs can alter primary cilium morphology; however, their impact on ciliary signaling remains unclear. This dataset explores the transcriptional changes induced by a-C and Grosheimin in RPE cells. RNA sequencing was performed to identify gene expression changes related to ciliogenesis, microtubule organization, and Hedgehog (Hh) signaling. These data contribute to understanding how structurally distinct SLs modulate ciliary function and may inform future studies on ciliopathies and tumor biology.