Organic electrochemical transistor aptasensor for interleukin-6 detection

Explanation of included data for following publication: ------------------------------------------------------------------------------------- Organic electrochemical transistor aptasensor for interleukin-6 detection Chiara Diacci (1,2), Bernhard Burtscher (1), Marcello Berto (2), Tero Petri Ruoko (1), Samuel Lienemann (1), Pierpaolo Greco (3,4), Magnus Berggren (1), Marco Borsari (5), Daniel T. Simon (1), Carlo A. Bortolotti (2), Fabio Biscarini (2,4) 1) Laboratory of Organic Electronics, Department of Science and Technology, ITN, Linköping University, 601 74, Norrköping, Sweden. 2) Dipartimento di Scienze della Vita, Università di Modena e Reggio Emilia, via Campi 103, 41125 Modena, Italy. 3) Department of Neuroscience and Rehabilitation, Università di Ferrara, Via Borsari 46, 44121 Ferrara, Italy. 4) Center for Translational Neurophysiology of Speech and Communication, Istituto Italiano di Tecnologia, via Fossato di Mortara 17-193, 44100 Ferrara, Italy. 5) Dipartimento di Scienze Chimiche e Geologiche, Università di Modena e Reggio Emilia, via Campi 103, 41125 Modena, Italy. ------------------------------------------------------------------------------------- Manuscript Figure 2: SEM pictures Figure 3: CV for aptamer deposition (Time, Voltage, Current) in 1µM aptamer solution from 0V to -1V, 50mV/s for 30 cycles Impedance data for AuNP/PEDOT:PSS electrode (before aptamer deposition) and after aptamer deposition (Aptamer/AuNP/PEDOT:PSS); (Freq/Hz, Re(Z)/Ohm, -Im(Z)/Ohm, |Z|/Ohm, Phase/deg) in ferricyanide 5mM Figure 4: Fluorescent images (LSM, as czi format) for 4a: AuNP/PEDOT:PSS (no Cy3-tag, control) 4b: Cy3-aptamer/AuNP/PEDOT:PSS (sample) 4c: Cy3-aptamer/PEDOT:PSS (no AuNP, control) Figure 5: Data sets of transfer curves for multiple OECTs for various concentrations of IL6/TNF One sheet refers to one OECT sample with either IL6 or TNF The first coloumn indicates the gate voltage and the others the drain current with various concentration except the second column (Tween) with is without any analyte VDS = -0.3V VGS swept from 0 to 0.6V Supporting Information: Figure S1: AuNP/PEDOT:PSS electrical characteristics S1a: CV in 5mM ferricyanide from -0.5V to 0.5V at 50mV/s S1b: Transconductance for bare Au, PEDOT:PSS in PBS 10mM S1c: Transfer curves in PBS 10mM Figure S2: FTIR-ATR measurements Raw exported files from the FTIR (Au+PEDOT, Au+DNA, Au+PEDOT+NP, Au+PEDOT+NP+DNA) as txt file. For Au+DNA the normalized with the maximum and for the others the 1010cm-1 peak used for normalization. This is shown in the ATR.xlsx file. Figure S3: IL6 detection on AuNP/PEDOT:PSS gate Transfer Charasteristics of the AuNP/PEDOT:PSS electrode with increasing IL6 concentration with VDS=-0.3V and VGS from 0V to 0.6V Figure S4: Transconductance of OECT based aptasensor Datasets used for S4 are identical with the ones from Figure 5 Figure S5: Impedance of functionalized device with IL6 Each sheet is for a IL6 concentration. The normalized OECT concentration is the same as in Figure 5.