Transcriptomic analysis of cellular senescence induced by ectopic expression of ATF6α in human breast cancer cells. Transcriptomic analysis of cellular senescence induced by ectopic expression of ATF6α in human breast cancer cells

To investigate the molecular mechanism that triggers endoplasmic reticulum (ER) stress-induced cellular senescence in breast cancer cells, we established MCF7 cell lines exposed to ATF6α ectopic expression. We then performed gene expression profiling analysis using data obtained from RNA-seq of 9 different cells at three time points. Overall design: Comparative gene expression profiling analysis of RNA-seq data for MCF-7 cells from three distinct time points (baseline, 6 hours, and 9 hours after ATF6α ectopic expression), using Illumina HiSeq X10 and NovaSeq 6000 platforms with paired-end 100 base sequencing.