Effect of different carbon sources on culture pH, growth rate, and chronological life span.

Cells were aged in synthetic complete medium supplemented with the appropriate carbon source and pH measurements were taken at both day 2 and day 4 of the experiment. Control concentrations for all carbon sources were tested at 2% w/v, except ethanol and glycerol which were tested at 3%. Moderate dietary restriction (DR) concentrations were at 0.5% for all sources except ethanol and glycerol, which were reduced to 1%. Survival integral (SI) values represent the area under the survival curve between day 2 and day 26.*Denotes a statistically significant increase SI in response to DR (p