CHO-DG44. Cricetulus griseus strain:CHO

Chinese hamster ovary (CHO) cells have chromosome number distribution. Previously, DG44-SC20 and DG44-SC39 cell lines with modal chromosome numbers 20 and 39 were isolated from parental CHO-DG44 cells, and IgG3 expressing cells named IgG3-SC20 and IgG3-SC39 were established, respectively. Even though all of the IgG3-SC20 clones and half of the IgG3-SC39 clones contained the same number of vector integration sites (one each), antibody productivities of IgG3-SC39 clones were higher than those in any of the IgG3-SC20 clones. In this study, we performed transcriptome analysis to investigate the characteristic of high producer cells with chromosome aneuploidy.