Embryonic exposure to heat impacts development time, adult morphology, and fecundity in pea aphids

Stress experienced during embryogenesis can have long-lasting effects. These effects may be particularly pronounced in hemimetabolous insects, given that they do not undergo body reorganization prior to adulthood. Here, we examined the effects of maternal exposure to different temperatures (19 °C, 22 °C, 25 °C, 28 °C, 31 °C) in live-bearing, asexual female pea aphids (Acyrthosiphon pisum). Specifically, we reared pea aphid mothers at these different temperatures, transferred their offspring to a common permissive temperature of 19 °C, and subsequently measured offspring developmental time, adult morphological traits, and fecundity. We found that pea aphids largely did not survive to adulthood at 31 °C, so we were not able to examine their offspring. For the remaining temperatures, offspring whose mothers were raised at 19-25 °C had largely similar trait values, while those whose mothers were raised at 28 °C developed significantly more slowly, had smaller body parts, and had lower fecun..., , # Data from: Embryonic exposure to heat impacts development time, adult morphology, and fecundity in pea aphids

Dataset DOI: [10.5061/dryad.sqv9s4nhz](https://doi.org/10.5061/dryad.sqv9s4nhz)

## Description of the data and file structure

Table S1 contains the data needed to recreate Figures 2 and 4 in the associated article. It contains the following columns:

Sample = unique pea aphid ID

Maternal Temperature = temperature in which the mother was raised

Temporal Replicate = 1 or 2; the experiment was repeated 2x

Spatial Replicate = different plants in the same incubator at the same temperature

Days in first, second, third, fourth instar = time spent at each stage

Days to adulthood = total time to adulthood

Phenotype = adult phenotype, winged (W) or wingless (WL); W individuals were not used for analyses

Total days to reproductive maturity = days until mother gave birth to first nymph

48 hr fecundity = how many nymphs mother gave birth to in first 48 hours of reproduction

Mat...,