Velvet genome assembly of HiSeq 2000 100bp paired-end reads. Ceratonova shasta 2015 draft genome assembly

Ceratonova shasta is a myxozoan parasite of salmonid fish. Here, we assembled a draft genome of C. shasta to use for future myxozoan 'omics studies. Mature parasite myxospores were obtained from intestinal tissue: the tissue was first macerated manually and filtered through a 45um pre-filter, then washed by suspending them in PBS and pelleting them twice, then purified spores using centrifugation in a Percol gradient. We used a Qiagen DNeasy kit (Animal Tissue Protocol) to extract total genomic DNA, then quantified using a Qbit photometer. DNA was sequenced on an Illumina HiSeq 2000 using 100bp paired-end reads. Reads were filtered for adapters, low quality reads, homopolymers, and contaminants, and assembled using Velvet.