Substrate binding and transport by the C. glabrata multidrug transporter Cdr1

Cdr1 is a membrane transporter, that confer resistance to pathogenic yeast by expelling drugs out of the cell. This mechanism of resistance is the main route of resistance to azoles, a family of compounds widely used in clinical settings. Infections of Candida species in immunocompromised patients is a major problem with compromised outcome for patients. We purify Cdr1 from C. glabrata, and got nice particle distribution on grids of this protein with different substrates and conditions of the transport mechanism. 600 movies data collection yielded a 5Ã reconstruction. We apply for time to get high resolution data