Transcriptome analysis of human 293T cells transfected with Ehhadh WT and different mutants (K329R, K335R, K344R, K572R). Transcriptome analysis of human 293T cells transfected with Ehhadh WT and different mutants (K329R, K335R, K344R, K572R)

We previously identified four lysine crotonylation (Kcr) sites at EHHADH (K329, K335, K344, K572). Ehhadh mutants in which lysine Kcr sites were simultaneously mutated to arginine by site-specific mutagenesis to mimic a decrotonylated status.To investigate the possible site-specific regulatory role of Ehhadh Kcr on biological processes, we performed transcriptome analysis on 293T cells that transfected with Ehhadh WT or mutants (K to R) recombinant Myc-tagged plasmids. Overall design: Examination the differentiation expressed genes (DEGs) in cells transfected with Ehhadh mutations (K329R, K335R, K344R, K572R) and WT