DAP-seq reveals FvSEP3 directly targets downstream genes relevant to strawberry fruit ripening

To better understand FvSEP3-mediated transcriptional regulation of fruit ripening, we performed DNA affinitypurification sequencing (DAP-seq) to uncover FvSEP3 directly binding sites at the genome level. For DAP-seq analysis, the recombinant FvSEP3 fusion protein was used to purify the sheared genomic DNA of strawberry fruits. Two independent biological replicates of DAP-seq and DNA 'input' negative control libraries were prepared and submitted for deep sequencing.