Amplicon-sequencing over the Anopheles gambiae CP, AP2 and Aper1 loci after non-autonomous gene drive

We co-opted the native regulatory sequences of three midgut-specific loci of the malaria vector Anopheles gambiae to host a prototypical antimalarial molecule and guide-RNAs encoded within artificial introns, that support efficient gene drive. We assessed the homing rate and resistance allele formation in male and female transhemizygotes, as well as in the G3 wild-type and the vasa-Cas9 controls.