Proteomic approach during the induction of somatic embryo-genesis in Coffea canephora

This research seeks to contribute to our understanding of the primary cellular pathways involved in the induction of SE in C. canephora. The process of our model consists of two stages: 1) preconditioning in MS medium with auxin (NAA) and cytokinin (KIN); and 2) induction in Yasuda liquid medium added with cytokinin (BA). Therefore, in this study, we analyzed different days of the SE induction process using shotgun label-free quantitative proteomics. 1630 proteins were found among different sampling days of the process, of which, the majority were up-accumulated during the induction stage. We found that some of the most enriched pathways during the process were the biosynthesis of amino acids and secondary metabolites. Eighteen proteins were found related to auxin homeostasis and two to cytokinin metabolism, such as ABC, BIG, ILR, LOG, and ARR, among others. Ten proteins and transcription factors related to SE were also identified, like SERK1, SKP1, nuclear transcription factor Y, MADS-box and calreticulin,; and 19 related to other processes of plant development, among which the 14-3-3 and PP2A proteins stand out.