Proteomic response of macrophages to human cancer Raji cells

In order to determine changes in phosphorylation events in macrophages upon phagocytosis of cancer cells, mouse macrophages were co-cultured with human Raji cancer cells. Macrophages were collected as 0, 1, 15, 60 and 120 minutes after incubation. Phosphosite abundances were compared between timepoints to determine differential phospho signaling over time.