GSF1635: RNA-seq data of OVCAR3-DAB2IP expressing cells vs. control

Ovarian cancer (OC) is the leading cause of death from gynecologic malignancies in the US. Ovarian cancer stem cells (OCSCs) have been shown to drive chemoresistance and tumor progression but the mechanism remains incompletely understood. Disabled Homolog 2- Interacting Protein (DAB2IP) is a potent tumor suppressor identified in multiple types of cancers and we demonstrate that DAB2IP is silenced by the repressive epigenetic mark H3K27me3 at the DAB2IP promoter loci in OCSCs. In triplicate,OVCAR3 cells were transfected with DAb2IP expressing vectors and empty vectors. RNA was harvested from the adipocytes using the RNAEasy kit (Qiagen). RNA-seq libraries were prepared using TruSeq Stranded mRNA Library Prep Kit (Illumina #RS-122-2101) according to manufacter's protocol.