Cucumis sativus cultivar:Ilan Raw sequence reads
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https://www.ncbi.nlm.nih.gov/sra/SRP553296
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Cucumis sativus L. cv. Ilan was used for CGMMV susceptibility analyses. Seeds were planted in P. spinosum-naturally contaminated commercial one-ton soil medium (Green 90 EvenAri, Beit Elazari, Israel) and -free soil (10 kg packs, (Green 90 EvenAri, Beit Elazari, Israel) and grown in a greenhouse with daytime addition of white fluorescent light, at 25C. Cucumber seedlings at the cotyledon stage with a small true leaf (about seven days post-emergence) were dusted with Carborundum (320 grit powder, Fisher Scientific, USA) before mechanical inoculation with virus-bearing sap (ca. 1:10 ratio g tissue/ml H2O) containing CGMMV. Cucumber was used as a source of inoculum for CGMMV. For the sequential soilless medium experiments, we used the 1-meter Coco Coir growbags (Pelemix, Israel). Following CGMMV inoculation, the plants were grown at 25C or 32C. Stems and roots were collected from wilting plants for oomycete and fungus analysis
创建时间:
2024-12-24



