Cells were dissociated into single cells with 30% TrypLE as described elsewhere in this study and then incubated with fluorophore-conjugated antibodies, designed against cell surface markers, diluted
MFI for MICAB1 mAb staining at the surface of Raji or C1R cells transfected with the indicated MICA alleles (*001, *004, *007, *008), MICB*002, ULBP1 or the empty vector (Neo). 19E9 = MICAB1 Antibo