Cell-density resolved comparative analysis of D. shibae wild-type vs. delta-luxI1

A quorum sensing null mutant was compared with D. shibae wild-type in order to identify quorum sensing controlled traits in this organism. Samples were taken at different optical densities in the exponential phase and in the stationary phase in order to clarify if quorum sensing regulation is dependent on cell-densitiy Samples from Dinoroseobacter shibae DFL 12 wild-type and delta-luxI1 (Dshi_0312) are compared during exponential (OD600 0.1, 0.2, 0.4, 0.6, 0.8) and stationary phase (6h post maximum OD600), two biological replicates