S5 Data -

(A) Traces of normalized FRET ratio during PDF application in l-LNvs from starved and fed Pdf-GAL4>UAS-Epac1 flies. (B)The amplitude of l-LNv responses to PDF in 5-day-old Pdf-GAL4>UAS-Epac1 flies following 21–24 h of starvation. Data are shown for 8 h bins. (C) Sleep in minute/hour in Cs flies maintained on a 12:12 LD schedule during sleep restriction day 2 and during recovery. (D) Traces of normalized FRET ratio during PDF application in l-LNvs recorded from Pdf-GAL4>UAS-Epac1 flies during sleep restriction sleep deprivation and bang. (F) The amplitude of l-LNv responses to PDF in l-LNvs during baseline, sleep restriction, and recovery. (G) Sleep rebound expressed as a difference with baseline sleep in Dcr2; c929-GAL4/UAS-PdfrRNAi, Dcr2; c929-GAL4/+, and UAS-PdfrRNAi/+ flies. FRET, Förster Resonance Energy Transfer; LD, light:dark; l-LNv, large ventral lateral neuron; PDF, pigment dispersing factor.
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