Trichoderma atroviride small RNA1 targets the Arabidopsis PRIM2 gene to establish a mutualistic relationship. Trichoderma atroviride small RNA1 targets the Arabidopsis PRIM2 gene to establish a mutualistic relationship

This study investigates the multifaceted defense mechanisms of plants in response to beneficial fungi Trichoderma atroviride. The researchers focus on a small RNA, Ta_sRNA1, produced by T. atroviride during its interaction with Arabidopsis thaliana. Overall design: A. thaliana roots inoculation with T. atroviride was performed and then collected from the interaction zone at 24, 48, 72, and 96 h of coculture. Total RNA was extracted and sRNA-seq libraries were generated according to the sRNA cloning protocol provided by Solexa.