Gene expression profile in tongue squamous carcinoma cells with stably transduced with LINC01011 shRNA under cisplatin treatment

chemoresistance frequently leads to therapeutic failure in oral squamous cell carcinoma (OSCC). Recently, studies have reported that lncRNAs play active regulatory roles in gene transcription and further regulate biological properties of cancer in this way. In this study, to further expore lncRNAs downstream target gene in regulating mitochondrial fission and cisplatin sensitivity in TSCC, we profiled the expression of gene in two TSCC cell lines(CAL-27 and SCC-9) after LINC01011 knockdown and treated with cisplatin treatment for 24h. We investigated the differential gene expression profiles in tongue squamous carcinoma cells between 6 groups: the CAL27 control group, the CAL27 stably transduced with shRNA2 targeted LINC01011 (CAL27 shRNA2 group), the CAL27 stably transduced with shRNA5 targeted LINC01011 (CAL27 shRNA5 group), the SCC9 control group and the SCC9 stably transduced with shRNA2 targeted LINC01011 (SCC9 shRNA2 group), the SCC9 stably transduced with shRNA5 targeted LINC01011 (SCC9 shRNA5 group). Sample labeling and array hybridization were performed by Human Gene expression 4×44K v2 microarrays according to the Agilent Whole Genome Oligo Microarrays protocol (Arraystar, Inc., Rockville, MD, USA). After quantile normalization of the raw data, the genes detected were chosen for further data analysis. Differentially expressed genes with statistical significance between the two groups were identified through P value/FDR filtering.