we evaluated the bias of nested PCR in estimating microbial diversity and community structure by comparing it with standard PCR. We investigated the influence of microbial diversity (relatively low vs
NanoCLUST (doi: 10.1093/bioinformatics/btaa900) is an analysis pipeline for the classification of amplicon-based full length 16S rRNA nanopore reads. This experiment contains one of the datasets (MOCK
The microbial assemblages of Lake Cisó and Lake Vilar (Banyoles, northeast Spain) were analyzed in space and time by microscopy and by performing PCR-denaturing gradient gel electrophoresis (DGGE) and
In this study, we examine several commonly used protocols for extracting bacterial DNA from ants to assess the biases inherent in each method. Bacterial community composition recovered using Illumina
The objective of this study is to establish standardized methodologies for efficient and reliable vaginal microbiome profiling. Vaginal samples were collected using three different swab types to compa