Determining the effects of hepatic As3mt overexpression on the response to arsenic in the liver of zebrafish larvae. Determining the effects of hepatic As3mt overexpression on the response to arsenic in the liver of zebrafish larvae

This study was carried out to compare changes in gene expression in 120 hpf livers that overexpress as3mt in hepatocytes between after 24-hour exposure to 1.0 and 1.5 mM iAs. Tg(fabp10a:As3mt) untreated = CL 1 AS3MT 0; Tg(fabp10a:As3mt) iAs treated = CL 1 AS3MT 1 or CL 1 AS3MT 1.5. Overall design: The samples in this series include only livers from 120 hpf zebrafish larvae that over express as3mt in hepatocytes with and without exposure to 1 and 1.5 mM of inoragnic arsenic (iAs) from 96 - 120 hpf. This study was carried out by comparing RNA extracted from 120 hpf zebrafish livers from six groups: untreated or 24-hour exposure to 1 mM or 1.5 mM iAs in CAAX-EGFP positive larvae and CAAX-EGFP positive and as3mt overexpressing larvae. To generate these samples, (tg:(fab10a:as3mt, cry:dsRed) were outcrossed to tg(fabp10a:CAAX-EGFP) and the larvae that only had the CAAX-EGFP expression in the liver and cry:dsRed in the eye were collected. RNA from 12 livers per group collected from 5 clutches (biological replicates) per group were extracted using Trizol/Chlorofom, PolyA selected and sequenced on Illumina NextSeq550.