Investigation of the functional contribution of Riplet in effector CD8 T cells

To understand the function of Riplet in effector CD8 T cells, we have employed whole genome microarray expression profiling of effector CD8 T cells from wild-type (WT) and Riplet knockout (KO) mice. Naive CD8 T cells isolated from splenocytes in WT and Riplet KO mice were stimulated with anti-CD3 and anti-CD28 Abs and after resting culture, restimulated with anti-CD3 Ab in vitro and mRNA was isolated. Effector CD8 T cells from WT and Riplet KO mice were stimulated with anti-CD3 Ab in vitro for 4 hours.