Single-cell transcriptomics during different phases post cardiac ischemia reveals secreted B2M to regulate fibroblast activation

The efficiency of the repair process following ischemic cardiac injury is a crucial determinant for the progression into heart failure and is controlled by both intra- and intercellular signaling within the heart. An enhanced understanding of this complex interplay will enable better exploitation of these mechanisms for therapeutic use. We used single-cell transcriptomics to collect gene expression data of all main cardiac cell types at different time-points after ischemic injury. Overall design: Single-cell transcriptomes were generated at several timepoints after ischemia-reperfusion injury in adult mice. Also, single-cell transcriptomes were generated 3 days after myocardial infarct surgery.