CIL:39786, Lytechinus pictus, epithelial cell, mesenchymal cell. In Cell Image Library
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https://library.ucsd.edu/dc/object/bb3044940v
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资源简介：
Images taken on a JEOL 35C SEM.
Processing protocol:
1. 2% GTA in 75% MBL SW or FilteredSW
FSW - fix for 1.5hrs. RT.
2. Rinse with 0.1M NaCacodylate buffer pH 7.4
3. 0.5% OSO4 in .075M NaCacodylate buffer pH 7.4 - 30' in the cold
4. Rinse in 0.1M NaCacodylate buffer ph 7.4
5. Dehydrate thru ETOH: 30%, 50%, 70%, 95% for 5 min each
6. 100% - 3 changes - 15' each
7. Critical Point Dry
8. Stick embryos down onto silver paint. Gently crack open with glass needle (pipette drawn out to a fine point).
9. Sputter coat with AuPd.
提供机构：
UC San Diego Library Digital Collections
创建时间：
2021-04-15
