Comprehensive systems biology analysis of a seven-month cigarette smoke inhalation study in C57BL/6 mice

Smoking of combustible cigarettes has a major impact on human health. Using a systems toxicology approach in a model of chronic obstructive pulmonary disease (C57BL/6 mice), we assessed the health consequences in mice of smoke derived from a prototype modified risk tobacco product (pMRTP) as compared to conventional cigarettes. We investigated physiological and histological endpoints in parallel with transcriptomics, lipidomics, and proteomics profiles in mice exposed to a reference cigarette (3R4F) smoke or a pMRTP aerosol for up to 7 months. We also included a cessation group and a switching-to-pMRTP group (after 2 months of 3R4F exposure) in addition to the control (fresh air-exposed) group, to understand the potential risk reduction of switching to pMRTP compared with continuous 3R4F exposure and cessation.
The dataset shared here describes the lipidomics analyses performed on lung, liver, and plasma of these mice at multiple time points. Lipids were characterized using, in addition to (untargeted) shotgun lipidomics, a set of targeted methods to specifically characterize sphingolipids, ceramides, triglycerides, eicosanoids, and gangliosides.