UV and visible fluorescence images of maize stem: macroscopy and confocal microscopy

Fluorescence Macroscopy is a full field imaging system at low magnification. The interest is to rapidly observe large fields of view (~5x5 mm²). Fluorescence filters are used that select excitation and emission ranges of wavelengths. The spectral resolution is low. Confocal microscopy equipped with a spectral detector and several excitation lasers allows to acquire hyperspectral images. The spectral resolution is around 6-10 nm. The field of view is small and is about 500 x500 µm² with a good spatial resolution. The fusion or the multiset analysis of the two kinds of images should provide a link between the two scales of observation and therefore a strategy to develop multiscale acquisition.