DDX5 competes with ZC3H12D regulating CCND1 transcript stability to modulate cell cycle progression in breast cancer

This study was performed according to protein pull-down assay using GFP-coated beads in MDA-MB-468 cells expressing GFP/ZC3H12D fusion protein, and then the precipitated proteins were isolated by running SDS-PAGE and silver staining, and identified by high resolution mass spectrometry.This study was performed according to RNA pull-down assay using biotin-labeled WT RNA probe (with stem-loop structure) or mutant RNA probe (without stem-loop structure) and streptavidin-coated Dynabeads in human breast tumor cell MDA-MB-468, and then the pulled down proteins were isolated by SDS-PAGE and silver staining, and identified by high resolution mass spectrometry.