Transcriptome alteration of cancer stem cells (CSCs) and non-CSCs when CSCs were depleted in HNSCC

To investigate the difference of signaling and cancer stemness activation molecules in CSCs and non-CSCs when Bmi1 was inhibited. Overall design: The data performed RNA-Seq were as follows: 1) HN6 ALDH+ CSCs treated with PTC209 and DMSO;2) K16+ tumor cells from tumor-bearing K16CreER; RosatdTomato mice treated with PTC209 versus control vehicle; 3) Epcam+ ALDH- tumor cells from PDX#3 treated with PTC209 versus control vehicle;