Zbtb46 regulated gene expression in ex vivo differentiated embryonic stem cells. Mus musculus strain:129/Ola

For this study we engineered murine Zbtb46-inducible embryonic stem cell lines and characterized their gene expression profile during ex vivo differentiation with RNA sequencing. Embryonic stem cell derived 5-day differentiated , magnetically separated Flk1+ cells were cultivated with or without Zbtb46 induction. To uncover the early impact of Zbtb46, cells were harvested after 24 hours; in addition, the transcriptomic profile was also determined after 72 hours.