Nos1ap constitutive null mice embryonic day 13.5 heart transcriptome

To assess the impact of Nos1ap loss on heart transcriptome during development, RNA-seq was performed in heart tissue from wildtype (males; n=5) and Nos1ap null homozygous (males; n=5) embryonic day 13.5 (e13.5) mice, and assessed for differential gene expression. At a false discovery rate of 1% and an absolute log2-fold change over 1, only two genes (Mt2 and Gm7694) were differentially expressed. These findings indicate that constitutive loss of Nos1ap has no major widespread impact on transcriptome in e13.5 hearts. Overall design: poly-A RNA-seq in embryonic day 13.5 mouse heart from five wildtype males and five Nos1ap constitutive null males.