Quantitative analysis of wild type and MeCP2-/y mice hippocampus miRNAs using next generation sequencing

To determine whether the miRNA expression profile is altered by MeCP2, we performed Solexa-based RNA sequencing(RNA-seq) to assess global changes in the expression pattern of miRNAs caused by the loss of MeCP2 in mecp2 null (knockout[KO]) mice (Chen et al., 2001). Hippocampus miRNA profiles of 30-day old wild type (WT) and MeCP2-/y mice were generated by deep sequencing using Illumina HiSeq 2000为探究MeCP2是否会改变microRNA (miRNA)表达谱，我们采用基于Solexa技术的RNA测序（RNA-seq）方法，检测了mecp2基因敲除（knockout[KO]）小鼠（Chen等，2001）中MeCP2缺失所引发的miRNA表达模式整体变化。本研究通过Illumina HiSeq 2000平台开展深度测序，获取了30日龄野生型（wild type[WT]）与MeCP2-/y小鼠的海马体miRNA表达谱。