RNA-seq for NF-YA-IR

Title / Project Name: Transcriptomic reprogramming during intestinal regeneration and upon NF-YA depletion in the Drosophila midgut Dataset Summary / Abstract: This dataset comprises bulk RNA-sequencing (RNA-seq) profiles generated from Drosophila melanogaster adult female midguts to investigate the global transcriptional networks regulating intestinal stem cell (ISC) activity, tissue regeneration, and dysplastic overgrowth. The dataset is structured around two major experimental designs: Intestinal Injury and Recovery Time-Course: To identify genes dynamically engaged during tissue repair, wild-type adult female flies were subjected to 3% dextran sodium sulfate (DSS) induced epithelial damage for 4 days, followed by a return to a normal diet. Dissected midguts were collected at four critical functional phases: early injury (DSS Day 1), late injury (DSS Day 4), early recovery (Recovery Day 1), and late recovery (Recovery Day 4), alongside parallel sucrose-fed control samples. Progenitor-Specific Genetic Manipulation: To identify the downstream transcriptional programs strictly controlled by the NF-Y complex, bulk RNA-seq was performed on midguts comparing control flies with those undergoing progenitor-specific knockdown of the rate-limiting subunit NF-YA (esgts > NF-YA-IR) after 10 days of transgene induction at 29°C.