Transcription profiling of mouse dermis from epithelial activated beta-catenin mutant embryo

Beta-catenin signaling is required for hair follicle development, but it is unknown whether it is sufficient to activate expression of hair follicle genes in embryonic skin. To address this we profiled gene expression in dermis from E15.5 KRT14-Cre Ctnnb1(Ex3)fl/+ embryos carrying an activating mutation in epithelial beta-catenin, and control littermate embryos. Experiment Overall Design: Total dermal RNA from two KRT14-Cre Ctnnb1(Ex3)fl/+ and two control littermate E15.5 embryos was hybridized to Affymetrix GeneChip Mouse Genome MOE430 2.0 oligonucleotide microarrays. Experiment Overall Design: Appended below is Table S2: Full list of differentially expressed genes in KRT14-Cre Ctnnb1(Ex3)fl/+ mutant compared with control littermate dermis at E15.5, including normalization and filter parameters. Fold change, listed in the second column, gives the ratio of normalized mutant : control transcript levels.Beta-catenin信号通路（beta-catenin signaling）对毛囊发育不可或缺，但目前尚不明确其是否足以激活胚胎皮肤内毛囊相关基因的表达。为解答这一科学问题，我们对携带上皮细胞Beta-catenin激活突变的E15.5 KRT14-Cre Ctnnb1(Ex3)fl/+胚胎的真皮组织进行了基因表达谱分析，并以同窝对照胚胎作为对照。实验总体设计：提取2例KRT14-Cre Ctnnb1(Ex3)fl/+突变胚胎与2例同窝对照胚胎的真皮总RNA，将其与Affymetrix GeneChip小鼠基因组MOE430 2.0寡核苷酸微阵列进行杂交。实验总体设计：下文附表格S2：E15.5时期KRT14-Cre Ctnnb1(Ex3)fl/+突变体真皮与对照真皮的差异表达基因完整列表，包含标准化与过滤参数。第二列标注的倍数变化（Fold change）为标准化后突变体与对照转录本水平的比值。