RNA sequencing reveals transcriptional changes after Hippo signaling activation in podocytes

Podocytes are crucial for the establishment and maintenance of the blood-urine filtration barrier in the glomeruli of the kidney. Activation of the Hippo signaling pathway in podocytes leads to the induction of apoptosis and could be linked to the pathogenesis of proteinuric diseases like focal segmental glomerulosclerosis (FSGS). To investigate the Hippo signaling controlled gene transcription, podocytes overexpressing LATS2, a core kinase of the Hippo pathway, were analyzed via RNA sequencing. The gene expression of 4 samples from a 8 h doxycyclin induced LATS2 overexpressing podocyte cell line was compared to 4 untreated samples by RNA sequencing.