Spatial metabolomics data of brain slices from 4T1 Pri mice, pre-LM mice and post-LM mice

<br>The extraction, detection, and quantitative analysis of metabolites in the samples were performed by Wuhan Metware Biotechnology Co., Ltd. Freshly dissected brains were collected from 4T1 Pri mice, pre-LM mice and post-LM mice, frozen in liquid nitrogen and embedded in 2% CMC. Frozen brains were sectioned to a thickness of 10 μm using a Leica CM1950 cryostat (Leica Microsystems GmbH, Wetzlar, Germany) and tissue sections were placed onto ITO-coated electrically conductive slides. 15 mg/mL DHB (2,5-dihydroxybenzoic acid) in acetonitrile: ddH₂O (9:1) were used for matrix coating and sprayed onto the glass slides by the TM-Sprayer (HTX Technologies). The MALDI-MSI experiments were performed using a TimsTOF fleX MS system (Bruker Daltonics, Germany) in positive mode. Laser power was set to 80% with a frequency of 10 kHz. The MALDI mass spectra data was acquired over a range of 50-1300 Da with 50 mm spatial resolution. Afterward, the tissue sections were stained with hematoxylin and eosin (H&amp;E) for spatial localization of the region of interest. The mass spectrometry image data underwent preprocessing by SCiLS Lab 2023c (Bruker Daltonik GmbH). Metabolite content data were processed using UV (unit variance scaling) and visualized using R package.